Accumulation kinetics of three Scirpentriol-based toxins in oats inoculated in vitro with isolates of Fusarium sporotrichioides and Fusarium poae
- Publikations-Art
- Zeitschriftenbeitrag
- Autoren
- Schollenberger M, Müller H-H, Liebscher M, Schlecker C, Berger M and Hermann W
- Erscheinungsjahr
- 2011
- Veröffentlicht in
- Toxins
- Band/Volume
- 3/
- DOI
- 10.3390/toxins3050442
- Seite (von - bis)
- 442-452
- Schlagworte
- Fusarium, oats, scirpentriol toxins, Toxigenicity, Trichothecenes
<p><span style="color: #000000;"><span style="font-family: ">Abstract: Autoclaved oats were inoculated with a strain of <i>Fusarium sporotrichioides </i><span style="mso-bidi-font-weight: bold;">or </span><i>Fusarium poae. </i>Moisture content of oats after inoculation was at 38%, incubation took place in standing culture at 28 °C. The A-type trichothecenes, 4,15-diacetoxyscirp</span><span style="font-family: ">enol (4,15-DAS), 15-monoacetoxyscirpenol (1</span><span style="font-family: ">5-MAS), and scirpentriol (SCIRP) were analyzed by GC/MS. For each strain, three culture flasks were harvested at 2</span><span style="font-family: ">-</span><span style="font-family: ">3 day intervals starting immediately after inoculation. Total incubation time was 42 days <i>(F. poae) </i>and 56 days <i>(F. sporotrichioides). </i>Following peak accumulation, 4,15-DAS decreased below the detection limit for both strains, 15-MAS decreased below this limit for the isolate of <i>F. sporotrichioides, </i>for the isolate of <i>F. poae </i>it decreased to a level markedly below the peak value. SCIRP, after having peaked, decreased to some extent for the strain <i>F. sporotrichioides, </i>with a significant <i>(P </i>= 0.0029) negative linear regression of toxin content against culture age during this period. The content of 15-MAS, and in part also of 4,15-DAS, decreased along with an increase of SCIRP. This sequential accumulation pattern suggests the successive induction of esteras</span><span style="font-family: ">es deacetylating 4,15-DAS and 1</span><span style="font-family: ">5-MAS, as we</span><span style="font-family: ">ll</span><span style="font-family: "> as of enzymes involved in the metabolization of the parent alcohol, SCIRP. The results may explain, at least in part, the somewhat higher incidence in </span><span style="font-family: ">naturally contaminated compounds reported in the literature for SCIRP compared to 4,15-DAS and 15-MAS.</span></span></p>