Application of high-performance liquid chromatography coupled to nuclear magnetic resonance spectrometry, mass spectrometry and bioassay for the determination of active saponins from Bacopa monniera Wettst.

Publikations-Art

Zeitschriftenbeitrag

Autoren

Renukappa, T.; Roos, G.; Klaiber, I.; Vogler, B.; Kraus, W.

Erscheinungsjahr

1999

Veröffentlicht in

J. Chromatogr., A

Band/Volume

847/1+2

ISBN / ISSN / eISSN

0021-9673

Seite (von - bis)

109-116

Reversed-phase high-performance liq. chromatog. sepn. coupled to (structurally informative) spectroscopic methods like NMR and MS and an efficient bioassay have been used to det. the active compds. from a crude fraction of Bacopa monniera. The fraction contg. a mixt. of saponins with closely related structures was found to show a significant anthelmintic activity against Caenorhabditis elegans (used as a model test organism for detg. anthelmintic activity). The activity was correlated to two dammarane type triterpenoidal saponins contg. at least three sugar units. The optimization of sepn. for 1 mg of the crude sample on column and the sensitivity of on-flow one- and two-dimensional NMR expts. to the high-mol.-mass compds. (Mr 890-930) has been demonstrated.