Biosynthesis of Vitamin B12 in anaerobic bacteria

Publikations-Art

Zeitschriftenbeitrag

Autoren

Munder, M.; Vogt, J. R. A.; Vogler, B.; Renz, P.

Erscheinungsjahr

1992

Veröffentlicht in

European Journal of Biochemistry

Band/Volume

204/

Seite (von - bis)

679 - 683

Expts. on the incorporation of erythrose and formate into the 5,6-dimethylbenzimidazole moiety of vitamin B12 are described. In 1 expt., a 1:1 mixt. of D-[1-13C]erythrose and D-[1-13C]threose was added to an E. limosum fermn. The vitamin B12 formed was methylated at N3 of its 5,6-dimethylbenzimidazole part and degraded to 1,5,6-trimethylbenzimidazole. The 13C-NMR spectrum of this compd. exhibited a single prominent signal at 109.5 ppm due to 13C labeling in C7. This shows that C1 of erythrose or threose was originally.