Effect of calf spleen and calf thymus extracts, thymopentin and tuftsin on phagocytic activity of polymorphonuclear leukocytes

Publikations-Art

Zeitschriftenbeitrag

Autoren

Krasowski, H.; Stehle, P.; Fuerst, P.; Kraus, W.

Erscheinungsjahr

1992

Veröffentlicht in

Arzneim.-Forsch.

Band/Volume

42/2

Seite (von - bis)

147-51

The in vitro influence of proteolyzed or untreated calf spleen and thymus exts., tuftsin, and thymopentin on the phagocytic activities of human polymorphonuclear leukocytes was investigated. At 200.mu.g/mL, all prepns. stimulated the leukocyte activity. The in vitro influence of proteolyzed or untreated calf spleen and thymus exts., tuftsin, and thymopentin on the phagocytic activities of human polymorphonuclear leukocytes was investigated. At 200.mu.g/mL, all prepns. stimulated the leukocyte activity measured by lucigenin-amplified chemiluminescence. This effect was considerable with tuftsin, thymopentin, and spleen ext., compared to the thymus prepns. No difference in chemiluminescence was obvious between hydrolyzed and untreated thymus exts. The phagocytosis of opsonized zymosan was investigated by light microscopy. Using identical substrate concns., only spleen ext. showed a stimulation. Polymorphonuclear leukocyte activities may be regulated by substrate-specific mechanisms on the cellular level. measured by lucigenin-amplified chemiluminescence. This effect was considerable with tuftsin, thymopentin, and spleen ext., compared to the thymus prepns. No difference in chemiluminescence was obvious between hydrolyzed and untreated thymus exts. The phagocytosis of opsonized zymosan was investigated by light microscopy. Using identical substrate concns., only spleen ext. showed a stimulation. Polymorphonuclear leukocyte activities may be regulated by substrate-specific mechanisms on the cellular level.