Transformation of 2-chloroquinoline to 2-chloro-cis-7,8-dihydro-7,8-dihydroxyquinoline by quinoline-grown resting cells of Pseudomonas putida 86
- Publikations-Art
- Zeitschriftenbeitrag
- Autoren
- Fetzner, S.; Vogler, B.; Lingens, F.
- Erscheinungsjahr
- 1993
- Veröffentlicht in
- FEMS Microbiol. Lett.
- Band/Volume
- 112/2
- Seite (von - bis)
- 151-7
Resting cells of P. putida strain 86 that had been grown on quinoline transformed 2-chloroquinoline (I) to 2-chloro-cis-7,8-dihydro-7,8-dihydroxyquinoline (II), which was not converted further. 7,8-Dioxygenating activity was present when the enzymes of quinoline catabolism were induced. Quinoline-grown cells of strain 86 treated simultaneously with I and D-(-)-threo-chloramphenicol to prevent protein biosynthesis also formed the cis-7,8-dihydrodiol of I. Succinate-grown resting cells did not oxidize I. Acid-catalyzed decompn. of II predominantly yielded 2-chloro-8-hydroxyquinoline. By analogy, accumulation of the putative dead-end metabolite 1H-8-hydroxy-2-oxoquinoline during growth of P. putida 86 on quinoline is suggested to likewise result from dehydration of the 7,8-dihydrodiol of 1H-2-oxoquinoline.