Detection of Phloridzin in Strawberries (Fragaria x ananassa Duch.) by HPLC-PDA-MS/MS and NMR Spectroscopy
- Publication Type
- Journal contribution (peer reviewed)
- Authors
- Hilt, P.; Schieber, A.; Yildirim, C.; Arnold, G.; Klaiber, I.; Conrad, J.; Beifuss, U.; Carle, R.
- Year of publication
- 2003
- Published in
- J. Agric. Food. Chem.
- Band/Volume
- 51/10
- DOI
- 10.1021/jf021115k
- Page (from - to)
- 2896-2899
The phenolic profile of strawberry fruits (Fragaria x ananassa Duch., Rosaceae) was investigated by high-performance liq. chromatog. with photodiode array detection. A peak displaying retention time and UV spectral data identical to those of phloridzin (phloretin 2'-O- -D-glucoside), a dihydrochalcone glucoside so far considered characteristic of apples, was monitored. For further characterization, crude exts. of strawberries were purified on polyamide, and the target compd. was isolated by preparative and anal. HPLC. Structure elucidation was performed on the basis of APCI- and ESI-MS in the neg. ion mode as well as by 1D and 2D NMR spectroscopy using authentic phloridzin for comparison. The D-configuration of the sugar moiety was established by HPLC anal. of the corresponding acyclic 1-deoxy-1-(N-acetyl-methylbenzylamino)alditol acetate. Apart from its chemotaxonomic relevance, this first report on the occurrence of phloridzin in strawberries is of particular interest for the authenticity control of strawberry products such as juices, jams, and fruit prepns. since phloridzin has so far been used for the detection of fraudulent admixts.