Elution order of quercetin glycosides from apple pomace extracts on a new HPLC stationary phase with hydrophilic endcapping
- Publication Type
- Journal contribution (peer reviewed)
- Authors
- Schieber, A.; Hilt, P.; Conrad, J.; Beifuss, U.; Carle, R.
- Year of publication
- 2002
- Published in
- J. Sep. Science
- Band/Volume
- 25/5/6
- DOI
- 10.1002/1615-9314(20020401)25:5/6<361::AID-JSSC
- Page (from - to)
- 361-364
A stationary phase with hydrophilic endcapping was used for the sepn. of seven quercetin (Q) glycosides by high-performance liq. chromatog. The elution order was established as Q 3-rutinoside, Q 3-galactoside, Q 3-glucoside, Q 3-xyloside, Q 3-arabinopyranoside, Q 3-arabinofuranoside, and Q 3-rhamnoside. Since a recent study had revealed that a com. available ref. compd. (Q 3-arap) was erroneously labeled as avicularin (Q 3-araf), stds. of the two isomers of Q 3-ara were characterized by 1D and 2D NMR spectroscopy, and by optical rotation. The predominant Q glycosides detected in apple pomace were Q 3-gal, Q 3-araf, Q 3-rha and Q 3-xyl, whereas considerably lower amts. of Q 3-glc and Q 3-arap were found. Total content of Q glycosides was approx. 900 mg/kg on a dry matter basis, indicating that apple pomace is a good source of natural antioxidants.