Identification and quantification of flavonoids and ellagic acid derivatives in therapeutically important Drosera species by LC-DAD, LC-NMR, NMR, and LC-MS.
- Publication Type
- Journal contribution (peer reviewed)
- Authors
- Zehl, M.; Braunberger, C.; Conrad, J.; Crnogorac, M.; Krasteva, St.; Vogler, B.; Beifuss,U.; Krenn, L.
- Year of publication
- 2011
- Published in
- Anal. Bioanal. Chem.
- Band/Volume
- 400/8
- DOI
- 10.1007/s00216-011-4690
- Page (from - to)
- 2565-2576
Droserae herba is a drug commonly used for treatment of convulsive or whooping cough since the seventeenth century. Because of the contribution of flavo- noids and ellagic acid derivatives to the therapeutic activity of Droserae herba, an LC-DAD method has been devel- oped for quantification of these analytes in four Drosera species used in medicine (Drosera anglica, D. intermedia, D. madagascariensis, and D. rotundifolia). During elabo- ration of the method 13 compounds, including three substances not previously described for Drosera species, were detected and unambiguously identified by means of extensive LC-MS and LC-NMR experiments and by off- line heteronuclear 2D NMR after targeted isolation. The most prominent component of D. rotundifolia and D. anglica, 2″-O-galloylhyperoside, with myricetin-3-O-β- glucopyranoside and kaempferol-3-O-(2″-O-galloyl)-β-galactopyranoside, were identified for the very first time in this genus. The LC-DAD method for quantification was thoroughly validated, and enables, for the first time, separation and precise analysis of these analytes in Droserae herba. Simple sample preparation and use of a narrow-bore column guarantee low cost and simplicity of the suggested system, which is excellently suited to quality control of the drug or herbal medicinal products containing this drug.