Isolation of carotenoids from plant materials and dietary supplements by high-speed counter-current chromatography
- Publication Type
- Journal contribution (peer reviewed)
- Authors
- Aman, R.; Carle, R.; Conrad, J.; Beifuss, U.; Schieber, A.
- Year of publication
- 2005
- Published in
- J. Chromatogr. A.
- Band/Volume
- 1074/1-2
- DOI
- 10.1016/j.chroma.2005.03.055
- Page (from - to)
- 99-105
Methods for the isolation of lipophilic pigments from crude exts. of plant materials (spinach and sweet corn) by high-speed counter-current chromatog. (HSCCC) were developed. Particular attention was given to (all-E)-lutein and (all-E)-zeaxanthin. However, the concomitant pigments neoxanthin, violaxanthin, and β-carotene as well as chlorophylls a and b were also considered. Furthermore, for the 1st time dietary supplements contg. lutein and zeaxanthin were also used as a source for the recovery of carotenoids. Due to their simple matrix (oily excipient in soft gelatine capsules), sample prepn. was facilitated and consumption of solvents was minimized. The carotenoids were characterized by 1H NMR spectroscopy, by LC/APcI-MS in the pos. ionization mode, and by UV-vis spectroscopy. Data showed that the target compds. were of high purity (90-93%). Lutein and zeaxanthin may be used as ref. substances for anal. purposes.